ligation independent cloning serial cloner

#Ligation independent cloning serial cloner full
#Ligation independent cloning serial cloner Pc
#Ligation independent cloning serial cloner series

Traditional restriction-enzyme/ligase-based strategies are often inefficient, while novel alternative methods can be less economical. Therefore, accurate, consistent, and efficient cloning methods have the potential to accelerate biological research. Particularly, when a species is amenable to transgenic manipulation, cloning enables detailed study of gene and protein function in vivo. Department of Plant Biotechnology and BioinformaticsĪbstract Molecular cloning is a vital step in much of today's plant biological research., Che-Yang Liao, Willy AM van den Berg , STING Millenium - web-based suite of programs for visualizing molecular structure and comprehensive structure analysis:ĬASTp - webserver for identification and measurements of surface accessible pockets as well as interior inaccessible cavitiesPlant functional genomics : methods and protocols. PARVATI anisotropic displacement parameters statisticsįADE and PADRE - Shape and Complementarity Server NCONT - (ccp4) analyses contacts between subsets of atoms in a PDB file VADAR (Volume, Area, Dihedral Angle Reporter) SURFACE and AREAIMOL (ccp4) -surface programsĬastP - Volume and surface calculation of pocket or cavity VOIDOO - look for interior cavities, volumes of particular cavities (like active sites) Monster - A tool for inferring non-covalant interactions in proteins Protein Interaction Server - to obtain surface calculations NACCESS - Surface area calculaitons, per residue, or atom LIGPLOT/LigPlus/DIMPLOT - Interactions around a ligand or interface SiteEngine - wesite to define a active site and then search the PDB for similar sites. PDBredo - a site where they re-refined everyone's structures

#Ligation independent cloning serial cloner Pc

ProtBud - PC program to evaluate multimer contacts and compare to related structures. PISA-Protein Interfaces, Surfaces and Assemblies (using PDB or new coordinates) SCOP - Structural Classification of Proteins MUSTANG - A MUtiple STructure AligNment alGorithm Indonesia - USF's biological sequence analysis (not installed) - multiple sequence alignments STRAP - Multiple Structural/Sequence Alignment POSA - Partial Order Structural Alignments MATRAS - MArkovian TRAnsition of Structure evolution - does multiple alignments STAMP - Structural Alignment of Multiple Proteins (several choices) SSM - Secondary Structure Matching - multiple PDB comparison and sequence alignments. MSDServer - Macromolecular Structure Database (Can provide %similarity cutoff to see entire structural family) VAST - (NCBI) Vector Alignment of Search Tool - protein structural neighbors

IP6-activated fusion proteins: maps, paper, protocol, Vector sequence (2000) A modular Set of Prokaryotic and Eukaryotic Expression Vectors Analytical Biochemistry 277, 109-120): pKA8 complete DNA sequence, pKA8 Cloning Map, Original Cloning Paper description

#Ligation independent cloning serial cloner series

Yeast Vectors: pYES2.0, pTF162, Stuff from Tim's lab about this series of vectorsīaculoVirus Vectors: pAcGP67-A for expression with a signal sequence for secretion PGEX Vectors: pGEXvectors, pGST-parallel, pGST-PP and pGST-TEV vectors from Steve (additional GST-PP and TEV vector sequences) Modified pET Vectors: pET11a-His(7)-PP, pET16b-TEV

#Ligation independent cloning serial cloner full

PET151-TOPO Cloning site map and full vector mapĭUET Vectors: pACYDuet-1, pCDFDuet-1, pCOLADuet-1, pRSFDuet-1, pETDuet-1 Novagen's pET vector manual (expression, toxcicity tips, etc) SLIC cloning, pGST-PP-GFP and pHIS-SUMO-GFP Ligation Independent Cloning (LIC) vectors from Mark Connelly: pMCSG10, pMCSG7, pMCSG17-20, pMCSG19, pMCSG21 Yeast 2-hybrid vectors (sundquist lab) pGADT7 and pGBKT7 PDEST14 pDEST15, pDEST17, pENTR/TEV/D-TOPO, MCS PENTRL1pLaclacZaL4, pENTRL1pLaclacZaR5, pENTR元pLactetL2, pENTRL5LacIL4, pENTRL5pLacSpecL2, pENTRR4pLacSpecR3